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Molecular Technician (level 2)


Perform complex technical work involving molecular analysis of human biological material in a variety of specialized laboratories. Steps are extensive and complex requiring manual manipulation, close monitoring and control, extensive calculations, plus careful and accurate work.


  • Perform complex technical work involved in preparing human biological material for specialized microscopic, cellular, serologic, molecular, genetic, and image or flow cytometric analysis. This may include any of the following operations, singly or in combination on submitted fluieds (including blood or body fluids), cytological specimens, or fixed and unfixed tissues: freeze, fix, dehydrate, embed, section, centrifuge, culture, harvest, cytospin, immunohistochemical stain, immunofluorescent stain, hybridization of cytologic sections or suspensions and/or isolation of lymphocyte subpopulations and/or genomic DNA.
  • Perform histocompatibility, cytogenetic, histologic, cytometric, immunohistochemical, immunofluorescent, serological, cellular, molecular, and/or cytologic procedures related to specialized microscopic, molecular, genetic, cellular, serological, electrophoretic, or cytometric analysis including assessing the quality of the sample for analysis.
  • Maintain files of any/all of the following as appropriate: slides, frozen specimens, blocks, cell preparations, micrographs, typing and antibody screening trays, DNA preparations, electrophoresis gel photos, results, and reports. Maintain neat and clean laboratory workspace.
  • Prepare stock solutions and reagents following standard operating procedures and quality control procedures. Record quality control of reagent preparation. Recognize and resolve problems or report to upper level personnel.
  • Perform routine equipment maintenance according to standard operating procedures. Record temperatures and equipment maintenance in accordance with internal and external regulatory agencies. Recognize instrument malfunctions and report to upper level personnel.
  • Assist in inventory maintenance by monitoring inventory and notifying upper level personnel of low inventory.
  • Maintain logbook or database; accession samples, order tests, and bill cases. Interact with physicians, nurses, and other healthcare professionals.
  • Perform proficiency testing to maintain compliance with internal and external regulatory agencies.
  • Attend staff meetings, continuing education, safety and compliance training. Remain current on standard operating procedures by reviewing and signing the laboratory manual annually. Follow departmental policies and procedures such as attendance and time reporting policies.
  • Provide technical guidance, instruction, and consultation to faculty, students, and staff members as assigned including training of new personnel.
  • Operate complex and/or computerized microscopic, molecular, and/or cytometric equipment to analyze prepared cellular and molecular specimens, calibrate and maintain instrumentation, and validate quality of assay.
  • Type results, reports, and addendums to reports and/or enter preliminary results into Cerner including making case specific comments pertaining to quality of specimens and/or interpretation of results.
  • Assist in the development of clinical assays and the validation of those assays including performance of testing and/or analysis.
  • Perform other related duties incidental to the work described herein.
  • Cytogenetics and Image Cytometry Laboratories:
    • Perform fluorescence in situ hybridization utilizing multi-color probes and microscopic analysis using specialized microscopic equipment and software.
    • Perform cytometric or karyotypic analysis of stained cytologic sections or suspensions using specialized microscopic and/or cytometric equipment.

Education and Experience:

  • BS degree in Biological Science or related field
  • Two years experience in Image Cytometry or Cytogenetics laboratory-OR- 3 years of general laboratory experience in addition to certification with a minimum of one year with relevant experience of specialty type.
  • Certification in one of the following is required:
    • HTL(ASCP) or HT(ASCP) or CLSp(CG)-NCA
  • Must be trained in at least half of the aspects of the Image Cytometry or Cytogenetics laboratory, and have documented competency/proficiency in these aspects of the specialty service.

 Skills and Abilities:

  • Act as the person in charge (aka General Supervisor) in the absence of the Supervisor/Manager, after attaining 1 yr of high complexity laboratory experience
  • Provide daily supervision and/or oversight of the laboratory operation and personnel performing testing and reporting test results.
  • Provide direct supervision of all high complexity testing.
  • Monitor testing to ensure that acceptable levels of analytic performance are maintained.
  • Training periods are long and will require reading literature related to the specialty to maintain current knowledge of these dynamic, rapidly evolving, fields.  


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